Composite

Part:BBa_K5306006:Design

Designed by: Iulia Beres   Group: iGEM24_MSP-Maastricht   (2024-09-28)


ANRA Pathway


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 7
    Illegal suffix found in sequence at 305
    Illegal EcoRI site found at 2226
    Illegal EcoRI site found at 2638
    Illegal EcoRI site found at 4150
    Illegal XbaI site found at 3063
    Illegal XbaI site found at 4074
    Illegal XbaI site found at 4418
    Illegal XbaI site found at 4575
    Illegal XbaI site found at 5111
    Illegal XbaI site found at 6461
    Illegal SpeI site found at 3710
    Illegal SpeI site found at 4404
    Illegal PstI site found at 776
    Illegal PstI site found at 5353
    Illegal PstI site found at 6862
    Illegal PstI site found at 7798
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 2226
    Illegal EcoRI site found at 2638
    Illegal EcoRI site found at 4150
    Illegal NheI site found at 2512
    Illegal NheI site found at 3288
    Illegal NheI site found at 4958
    Illegal NheI site found at 5462
    Illegal NheI site found at 6491
    Illegal NheI site found at 8579
    Illegal SpeI site found at 306
    Illegal SpeI site found at 3710
    Illegal SpeI site found at 4404
    Illegal PstI site found at 320
    Illegal PstI site found at 776
    Illegal PstI site found at 5353
    Illegal PstI site found at 6862
    Illegal PstI site found at 7798
    Illegal NotI site found at 13
    Illegal NotI site found at 313
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 2226
    Illegal EcoRI site found at 2638
    Illegal EcoRI site found at 4150
    Illegal BglII site found at 2045
    Illegal BglII site found at 5385
    Illegal BglII site found at 6800
    Illegal BamHI site found at 4412
    Illegal XhoI site found at 2947
    Illegal XhoI site found at 9991
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 7
    Illegal suffix found in sequence at 306
    Illegal EcoRI site found at 2226
    Illegal EcoRI site found at 2638
    Illegal EcoRI site found at 4150
    Illegal XbaI site found at 3063
    Illegal XbaI site found at 4074
    Illegal XbaI site found at 4418
    Illegal XbaI site found at 4575
    Illegal XbaI site found at 5111
    Illegal XbaI site found at 6461
    Illegal SpeI site found at 3710
    Illegal SpeI site found at 4404
    Illegal PstI site found at 776
    Illegal PstI site found at 5353
    Illegal PstI site found at 6862
    Illegal PstI site found at 7798
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 7
    Illegal EcoRI site found at 2226
    Illegal EcoRI site found at 2638
    Illegal EcoRI site found at 4150
    Illegal XbaI site found at 22
    Illegal XbaI site found at 3063
    Illegal XbaI site found at 4074
    Illegal XbaI site found at 4418
    Illegal XbaI site found at 4575
    Illegal XbaI site found at 5111
    Illegal XbaI site found at 6461
    Illegal SpeI site found at 306
    Illegal SpeI site found at 3710
    Illegal SpeI site found at 4404
    Illegal PstI site found at 320
    Illegal PstI site found at 776
    Illegal PstI site found at 5353
    Illegal PstI site found at 6862
    Illegal PstI site found at 7798
    Illegal NgoMIV site found at 2314
    Illegal AgeI site found at 869
    Illegal AgeI site found at 2327
    Illegal AgeI site found at 9365
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

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References